Overview

The applications on Polly are built to process and visualize experimental data ranging from mass-spec based omics to sequencing-based omics, from dual-mode data visualization to the analysis of CRISPR screening. Though despite the variety and specificity of the apps present on Polly, there are a couple of features common across all apps. These features are built to help you get started with minimal effort, analyze and share results and processed data with your collaborators with ease.

Demo Data

To help you get started even if you do not have your own data, every app on Polly has demo data uploaded as shown in Figure 1, Figure 2 and Figure 3. Moreover the demo data also serves as a reference point for the input files and their format required for each app.

Figure 1. Demo Data for FirstView

Figure 2. Demo data for MetScape

Figure 3. Demo data for Labeled LC-MS app

Upload Data

All Polly applications provide you the option to upload data from local storage as shown in Figure 4 and Figure 5.

Figure 4. Upload data from local storage for MetScape

Figure 5. Upload data from local storage for Labeled LC-MS app

Some of the Polly applications also provide the ability to uplaod input files from Polly Project Files by using the option Import from Polly as shown in Figure 6. This simplifies data processing as well as makes Polly the platform where biological data can be stored and processed conveniently.

Figure 6. Upload data from project files for Labeled LC-MS app

Restore Analysis

All Polly applications contain the restore functionality that allows any analysis to be restored to the last step. Some of the Polly applications also provide the ability to save at that exact moment of your choice using the Save the State option. Analyses can be restored by navigating to the Analysis section of a project. Click on History for the specific analysis to restore. Clicking on Restore Analysis will take you back to the application with the same data used before as shown in Figure 5. For the applications providing the Save the state option, you have an additional step to choose which step to restore. Multiple states, if saved are depicted as a branch structure which makes it extremely easy to visually identify the state of interest as shown in Figure 8 and Figure 9. Restore helps laboratories and organizations with standardization across labs and improves reproducibility.

Figure 7. Restore for Angular applications

Figure 8. Restore for Shiny applications

Figure 9. Branch structure in case of multiple states saved during a single analysis for Shiny applications

Download Plots & Output

All applications on Polly allow you to download processed data and plots when displayed so that if needed you can verify from a third party about exactly how the data is being processed at a specific step. The tabular data can be downloaded as .csv files whereas the plots can be downloaded as .png, .jpeg or .svg files as shown in Figure 10, FIgure 11 and Figure 12.

Figure 10. Download Pathway Dashboard as an image or the processed data as a .csv file

Figure 11. Download PCA Plot as an image

Figure 12. Download the processed data as a .csv file

As with upload, data can be downloaded to local storage for Angular and Shiny apps as well as Polly Project Files for Shiny apps.

Reports

Reports serve as the culmination of each analysis where all plots and insights generated are added in a way that is easy to understand and draw conclusions from when shared with collaborators or even used as a point of reference for something done earlier. Presence of reports for each analysis ordered sequentially promotes reproducibility across the laboratory or the organization and reduces the manual labor involved in knowledge transfer.

To ensure that you do not spend a lot of time downloading plots and arranging them in a pre-defined order with the insights generated each time for a study, we have built One Click Report (OCR), functionality that allows you to generate reports in a standardized format.

Customization

The major advantage with our implementation is customization and ease of use. With our framework, we can implement an OCR for you on the app of your choice within 1 week. You can customize the structure of the report, any text to be included and the plots themselves. Plots not on the app can be added as well as the existing plots modified according to your requirements.

Currently, custom OCR is integrated in the following apps:

• MetScape